[Effect of supplement energy and nourish lung herbs on drug-resistance lung cancer with mechanisms of mitochondrial and caspase signal].

Abstract

OBJECTIVE:

To explore the effect of supplement energy and nourish lung (SENL) herbs on the growth of drug-resistance lung cancer cells, expression of multidrug resistance-associated protein (MRP) and mechanisms of mitochondrial and Caspase signal.

METHODS:

Drug-resistance lung cancer cell line SW1573/2R120 was treated with SENL herbs and Adriamycin. Cellular toxicity with MTT colorimetric assay, expression of MRP protein with flow microscopy, the fluorescence intensity of mitochondria membrane potetional and Ca2+ with laser confocal microscopy, the activities of caspase-3 and caspase-8 with colorimetric assay were detected.

RESULTS:

Compared with control group, cellular inhibitory rate was increased obviously, expression of MRP protein was decreased, mitochondria membrane potetional and intracellular Ca2+ were decreased, the activities of caspase-3 and caspase-8 were increased in the group of SENL herbs.

CONCLUSION:

SENL herbs can interfere and reverse drug resistance in lung cancer by the mechanism of decreasing mitochondria membrane potetional and increasing the activities of caspase-3 and caspase-8.

• Authors: Meng Xu, Xin-Hai Zhu, Liang-He Sheng
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 31 Issue 9 Pg. 1396-9 (Sep 2008) ISSN: 1001-4454 [Print] China
• PMID: 19180968 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drug Combinations
• Drugs, Chinese Herbal
• Multidrug Resistance-Associated Proteins
• Doxorubicin
• Caspase 3
• Caspase 8
• Calcium

Topics

• Antineoplastic Agents (administration & dosage, pharmacology)
• Calcium (metabolism)
• Caspase 3 (metabolism)
• Caspase 8 (metabolism)
• Cell Line, Tumor
• Doxorubicin (administration & dosage, pharmacology)
• Drug Combinations
• Drug Resistance, Neoplasm
• Drugs, Chinese Herbal (administration & dosage, pharmacology)
• Flow Cytometry
• Humans
• Lung Neoplasms (metabolism, pathology)
• Membrane Potential, Mitochondrial (drug effects)
• Multidrug Resistance-Associated Proteins (metabolism)
• Plants, Medicinal (chemistry)