[The anti-tumour effect of Wuxing soup and its mechanism in inducing apoptosis of tumour cells mediated by calcium].

Abstract

OBJECTIVE:

To confirm the anti-cancer effect and mechanism of Wuxing soup.

METHODS:

Inhibition of cellular growth under Wuxing soup treatment was observed by MTT; Apoptosis was detected by gel electrophoresis, transmission electron microscopy and FACS; The concentration of calcium was measured by fluorescence probe.

RESULTS:

After SGC-7901 cell being treated by Wuxing soup, it showed that: 1) Wuxing soup could specifically inhibit cancer cells proliferation in a time and dose dependent manner; 2) Typical apoptotic morphological changes and DNA ladder of SGC-7901 cells were observed; 3) calcium inhibitor Bapta AM could reduce the apoptotic rate and protect SGC-7901 cells in a dose dependent manner.

CONCLUSION:

Wuxing soup has an effective inhibition on cancer cells, and can induce SGC-7901 cells to apoptosis by calcium.

• Authors: Fei Mo, Jing-Ying Hu, Yu Gan, Yang-Xing Zhao, Xin-Tai Zhao
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 31 Issue 9 Pg. 1368-72 (Sep 2008) ISSN: 1001-4454 [Print] China
• PMID: 19180961 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Drug Combinations
• Drugs, Chinese Herbal
• Calcium

Topics

• Antineoplastic Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Arctium (chemistry)
• Calcium (metabolism)
• Cell Division (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Daucus carota (chemistry)
• Dose-Response Relationship, Drug
• Drug Combinations
• Drugs, Chinese Herbal (isolation & purification, pharmacology)
• Flow Cytometry
• Humans
• Plants, Edible (chemistry)
• Raphanus (chemistry)
• Stomach Neoplasms (pathology)
• Time Factors
• Tumor Cells, Cultured