M-PEIs nanogels: potential nonviral vector for systemic plasmid delivery to tumor cells.

Abstract

Successfully systemic gene therapy has been hindered by vector-related limitations, including toxicity and inefficient gene delivery to tumor cells after intravenous administration. In this study, we evaluated the potential of spherical polyethylenimine nanogels (M-PEIs) as a novel vector for intravenous delivery of plasmids to tumor cells. M-PEIs provided a sustained release of plasmids up to 14 days and were also effective in protecting plasmids from enzymatic degradation in serum-conditioned media. M-PEIs showed no obvious cytotoxicity to mammalian cells in vitro as well as to liver, heart and kidney in mice after intravenous injection. Importantly, following intravenous administration of M-PEIs/plasmid complexes into human hepatocellular carcinoma xenograft-bearing mice, green fluorescence protein reporter gene expression was predominantly found in the tumor. This study indicates that M-PEIs may be a candidate for systemic delivery of plasmids into tumors.

• Authors: L Dong, H Xu, Y-B Liu, B Lu, D-M Xu, B-H Li, J Gao, M Wu, S-D Yao, J Zhao, Y-J Guo
• Journal: Cancer gene therapy (Cancer Gene Ther) Vol. 16 Issue 7 Pg. 561-6 (Jul 2009) ISSN: 1476-5500 [Electronic] England
• PMID: 19180143 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nanogels
• polyethylene glycol polyethyleneimine nanogel
• Green Fluorescent Proteins
• Polyethylene Glycols
• Polyethyleneimine

Topics

• Animals
• Blotting, Western
• Cell Line, Tumor
• Cell Survival
• Genetic Therapy (methods)
• Genetic Vectors (chemistry)
• Green Fluorescent Proteins (genetics, metabolism)
• Humans
• Mice
• Nanogels
• Polyethylene Glycols (chemistry)
• Polyethyleneimine (chemistry)