Effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats using microarray technology.

Abstract

OBJECTIVES:

We have used microarray technology to detect the effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats.

METHODS:

Male Sprague-Dawley rats (180-200 g) were randomly divided into three groups: sham-operated; coronary artery ligation; and coronary artery ligation plus administration of Guanxin No.2 decoction (10.0 g raw materials/kg per day by gavage). The experiment was carried out on day seven after ligation.

KEY FINDINGS:

We found that the gene expression using microarray technology showed many differences in the border infarcted left ventricular area compared with the remote noninfarcted left ventricular area after administration of Guanxin No.2 decoction.

CONCLUSIONS:

Guanxin No.2 decoction has a long history in treating ischaemic cardiomyopathy in China, but the molecular mechanism has been unclear. In this study we found that some important genes may have contributed to the cardioprotective effect of Guanxin No.2 decoction.

• Authors: Xiaowei Zeng, Jun Yang, Xianzhe Yang, Dongsheng Hong, Limao Wu, Jun Yu
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 61 Issue 2 Pg. 213-9 (Feb 2009) ISSN: 0022-3573 [Print] England
• PMID: 19178769 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzaldehydes
• Benzoates
• Benzofurans
• Bridged-Ring Compounds
• Cardiotonic Agents
• Catechols
• Coumaric Acids
• Drugs, Chinese Herbal
• Glucosides
• Hydroxybenzoates
• Lactates
• Monoterpenes
• Plant Extracts
• Quinones
• guanxin No. 2
• hydroxysafflor yellow A
• peoniflorin
• protocatechuic acid
• 3,4-dihydroxyphenyllactic acid
• protocatechualdehyde
• Chalcone
• ferulic acid
• salvianolic acid B
• chuangxiong extract

Topics

• Animals
• Benzaldehydes (chemistry, isolation & purification)
• Benzoates (chemistry, isolation & purification)
• Benzofurans (chemistry, isolation & purification)
• Bridged-Ring Compounds (chemistry, isolation & purification)
• Cardiotonic Agents (chemistry, pharmacology, therapeutic use)
• Carthamus tinctorius (chemistry)
• Catechols (chemistry, isolation & purification)
• Chalcone (analogs & derivatives, chemistry, isolation & purification)
• Coumaric Acids (chemistry, isolation & purification)
• Dalbergia (chemistry)
• Drugs, Chinese Herbal (chemistry, pharmacology, therapeutic use)
• Gene Expression (drug effects)
• Gene Expression Profiling
• Glucosides (chemistry, isolation & purification)
• Heart Ventricles (drug effects, metabolism)
• Hydroxybenzoates (chemistry, isolation & purification)
• Lactates (chemistry, isolation & purification)
• Ligusticum
• Male
• Monoterpenes
• Myocardial Infarction (drug therapy, genetics, metabolism)
• Oligonucleotide Array Sequence Analysis (methods)
• Paeonia (chemistry)
• Plant Extracts (chemistry)
• Quinones (chemistry, isolation & purification)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Salvia miltiorrhiza (chemistry)