Current techniques for the study of troponin I phosphorylation in human heart.

Abstract

Cardiac troponin I phosphorylation has been shown to be reduced in human heart failure and hypertrophic cardiomyopathy using the increasingly popular Pro-Q Diamond phosphoprotein gel stain. In this brief report I discuss the use of Western immunoblotting, non-equilibrium isoelectric focussing and Pro-Q Diamond and introduce phosphate affinity SDS-PAGE using Phos-tag-acrylamide for the investigation of troponin I phosphorylation.

• Authors: Clare E Gallon
• Journal: Journal of muscle research and cell motility (J Muscle Res Cell Motil) Vol. 29 Issue 6-8 Pg. 169-72 ( 2008) ISSN: 1573-2657 [Electronic] Netherlands
• PMID: 19172403 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)

Chemical References

• Troponin I

Topics

• Electrophoresis, Polyacrylamide Gel (methods)
• Humans
• Myocardium (chemistry, metabolism)
• Phosphorylation
• Troponin I (metabolism)