STD sensor based on nucleic acid functionalized nanostructured polyaniline.

Abstract

STD (sexually transmitted disease, Gonorrhoea) sensor based on nucleic acid probe (from Opa, a multi-copy gene of Neisseria gonorrhoeae) functionalized nanostructured-polyaniline coated onto indium-tin-oxide-coated glass plate has been fabricated using avidin-biotin as cross-linking agent. This DNA functionalized electrode can specifically detect upto 0.5 x 10(-15)M of complementary target within 60s of hybridization time at 25 degrees C by differential pulse voltammetry (DPV) using methylene blue as electro-active DNA hybridization indicator. This highly sensitive and specific nucleic acid functionalized nanostructured-polyaniline electrode can distinguish presence of N. gonorrhoeae from Neisseria meningitidis and Escherichia coli culture and spiked samples from the urethral swabs of the patients.

• Authors: Renu Singh, Rachna Prasad, G Sumana, Kavita Arora, Seema Sood, R K Gupta, B D Malhotra
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 24 Issue 7 Pg. 2232-8 (Mar 15 2009) ISSN: 1873-4235 [Electronic] England
• PMID: 19162465 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aniline Compounds
• DNA, Bacterial
• polyaniline

Topics

• Aniline Compounds (chemistry)
• Biosensing Techniques (instrumentation)
• Colony Count, Microbial (instrumentation)
• DNA, Bacterial (analysis, genetics)
• Electrochemistry (instrumentation)
• Equipment Design
• Equipment Failure Analysis
• Gonorrhea (diagnosis, microbiology)
• Humans
• Male
• Nanostructures (chemistry, ultrastructure)
• Nanotechnology (instrumentation)
• Neisseria gonorrhoeae (genetics, isolation & purification)
• Oligonucleotide Array Sequence Analysis (instrumentation)
• Reproducibility of Results
• Sensitivity and Specificity