Human cells transformed in vitro by human cytomegalovirus: tumorigenicity in athymic nude mice.

Abstract

Athymic nude mice were inoculated with human embryo lung cells transformed in vitro by human cytomegalovirus (CMV). Of the inoculated animals, 62% developed tumors after an average latent period of 19 days. The tumors were composed of small, polygonal cells with large nulei and scanty cytoplasm embedded in an abundant collagenous matrix. The cells were poorly differentiated but may have been of epithelial origin. Adjacent structures were rarely invaded. CMV-related intracellular and membrane antigens were detected by indirect and anticomplement immunofluorescence techniques in cells cultured in vitro from the tumors.

• Authors: L Geder, J Kreider, F Rapp
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 58 Issue 4 Pg. 1003-9 (Apr 1977) ISSN: 0027-8874 [Print] United States
• PMID: 191621 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Antigens, Viral
• Cytochalasin B

Topics

• Animals
• Antigens, Neoplasm
• Antigens, Viral
• Cell Division (drug effects)
• Cell Nucleus (drug effects)
• Cell Transformation, Neoplastic
• Cells, Cultured
• Chromosome Aberrations
• Contact Inhibition
• Cytochalasin B (pharmacology)
• Cytomegalovirus (immunology)
• Humans
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Neoplasms, Experimental (genetics, immunology, pathology)
• Transplantation, Heterologous