Differential effects of triterpene glycosides, frondoside A and cucumarioside A2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells.

Abstract

Frondoside A is a pentaoside having an acetyl moiety at the aglycon ring and xylose as a third monosaccharide residue. Cucumarioside A(2)-2 is a pentaoside having glucose as a third monosaccahride unit. We compared the effects of frondoside A and A(2)-2 for cell death-inducing capability with close attention paid to structure-activity relationships. Both frondoside A and A(2)-2 strongly induced apoptosis of leukemic cells. Frondoside A-induced apoptosis was more potent and rapid than A(2)-2-induced apoptosis. A(2)-2-induced but not frondoside A-induced apoptosis was caspase-dependent. This suggests that holothurians may induce apoptosis of leukemic cells caspase-dependently or -independently, depending on the holothurian structure.

• Authors: Jun-O Jin, Valeria V Shastina, Sung-Won Shin, Qi Xu, Joo-In Park, Valery A Rasskazov, Sergey A Avilov, Sergey N Fedorov, Valentin A Stonik, Jong-Young Kwak
• Journal: FEBS letters (FEBS Lett) Vol. 583 Issue 4 Pg. 697-702 (Feb 18 2009) ISSN: 1873-3468 [Electronic] England
• PMID: 19162021 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A5
• Fluorescent Dyes
• Glycosides
• Saponins
• Triterpenes
• cucumarioside A2-2
• frondoside A
• cucumarioside
• Caspases
• Fluorescein-5-isothiocyanate

Topics

• Animals
• Annexin A5 (metabolism)
• Apoptosis (drug effects)
• Caspases (metabolism)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Enzyme Activation (drug effects)
• Fluorescein-5-isothiocyanate (metabolism)
• Fluorescent Dyes (metabolism)
• Glycosides (chemistry, isolation & purification, pharmacology)
• HL-60 Cells
• Humans
• Lethal Dose 50
• Leukemia (pathology)
• Molecular Structure
• Saponins (chemistry, isolation & purification, pharmacology)
• Sea Cucumbers (chemistry)
• Time Factors
• Triterpenes (chemistry, isolation & purification, pharmacology)