Prunella vulgaris extract and rosmarinic acid suppress lipopolysaccharide-induced alteration in human gingival fibroblasts.

Abstract

Periodontitis is a chronic disease associated with inflammation of the tooth-supporting tissues. The inflammation is initiated by a group of gram-negative anaerobic bacteria. These express a number of irritating factors including a lipopolysaccharide (LPS), which plays a key role in periodontal disease development. Plant extracts with anti-inflammatory and anti-microbial properties have been shown to inhibit bacterial plaque formation and thus prevent chronic gingivitis. In this study we tested effects of Prunella vulgaris L. extract (PVE; 5, 10, 25microg/ml) and its component rosmarinic acid (RA; 1microg/ml) on LPS-induced oxidative damage and inflammation in human gingival fibroblasts. PVE and RA reduced reactive oxygen species (ROS) production, intracellular glutathione (GSH) depletion as well as lipid peroxidation in LPS-treated cells. Treatment with PVE and RA also inhibited LPS-induced up-regulation of interleukin 1beta (IL-1beta), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and suppressed expression of inducible nitric oxide synthase (iNOS). The results indicate that PVE and RA are able to suppress LPS-induced biological changes in gingival fibroblasts. The effects of PVE and RA are presumably linked to their anti-inflammatory activities and thus use of PVE and RA may be relevant in modulating the inflammation process, including periodontal disease.

• Authors: A Zdarilová, A Svobodová, V Simánek, J Ulrichová
• Journal: Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 23 Issue 3 Pg. 386-92 (Apr 2009) ISSN: 0887-2333 [Print] England
• PMID: 19159670 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Cinnamates
• Depsides
• Lipopolysaccharides
• Plant Extracts
• Reactive Oxygen Species
• Glutathione
• rosmarinic acid

Topics

• Antioxidants (analysis, pharmacology)
• Cell Survival (drug effects)
• Cells, Cultured
• Cinnamates (analysis, pharmacology)
• Depsides (analysis, pharmacology)
• Drug Antagonism
• Fibroblasts (drug effects, metabolism, pathology)
• Gingiva (drug effects, metabolism, pathology)
• Glutathione (metabolism)
• Humans
• Lipopolysaccharides (antagonists & inhibitors, toxicity)
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Prunella (chemistry)
• Reactive Oxygen Species (metabolism)