[Establishment of human embryonic stem cell line NJGLLhES1].

Abstract

OBJECTIVE:

To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1.

METHODS:

From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas.

RESULTS:

NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas.

CONCLUSION:

A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.

• Authors: Hai-Xiang Sun, Xia Zhao, Ya-Li Hu, Ning-Yuan Zhang, Bin Wang, Hua Chen, Jun-Xia Wang, Zhi-Peng Xu, Lin-Jun Chen
• Journal: Zhonghua nan ke xue = National journal of andrology (Zhonghua Nan Ke Xue) Vol. 14 Issue 12 Pg. 1083-9 (Dec 2008) ISSN: 1009-3591 [Print] China
• PMID: 19157227 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Cell Culture Techniques
• Cell Differentiation
• Cell Line
• Embryo, Mammalian (cytology)
• Embryonic Stem Cells (cytology)
• Humans
• Mice
• Mice, Inbred ICR