Two-color immunofluorescence and flow cytometric analysis of lamina propria lymphocyte subsets in ulcerative colitis and Crohn's disease.

Abstract

By using two-color immunofluorescence with fluorescein isothiocyanate (FITC) and phycoerythrin (PE)-labelled monoclonal antibodies and multiparameter flow cytometry, we investigated lamina propria lymphocyte subsets of patients with ulcerative colitis (UC) and Crohn's disease (CD). Leu-3/Leu-2 (CD4/CD8) ratio of lamina propria lymphocytes (LPL) of CD (mean +/- SD: 1.9 +/- 0.8, P less than 0.01) was significantly decreased compared with controls (3.3 +/- 1.1), because of an increased number of CD8+ lymphocytes. The majority of lamina propria CD4+ cells were CD4+, Leu-8- and CD4+, CD45R- both in controls and IBD tissue. Many lamina propria T lymphocytes were activated, expressing HLA-DR antigen not only in IBD but also in controls. NK cells defined by CD16 and CD 56 (3.0 +/- 1.4%, P less than 0.01) were significantly decreased in patients with UC compared with controls (6.5 +/- 3.0%). A low proportion of B cells in the intestinal mucosa expressed Leu-8 antigen and CD23 antigen. The proportion of activated B cells of LPL was high in IBD mucosa as well as normal mucosa. These findings suggest that local activation of B cells leads to the loss of the expression of Leu-8 antigen and CD23.

• Authors: M Senju, K C Wu, Y R Mahida, D P Jewell
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 36 Issue 10 Pg. 1453-8 (Oct 1991) ISSN: 0163-2116 [Print] United States
• PMID: 1914770 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Monoclonal
• B-Lymphocyte Subsets (pathology)
• CD4-CD8 Ratio
• Colitis, Ulcerative (diagnosis, pathology)
• Crohn Disease (diagnosis, pathology)
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Humans
• In Vitro Techniques
• Intestinal Mucosa (pathology)
• Male
• Middle Aged
• Phenotype
• T-Lymphocyte Subsets (pathology)