Ginsenoside Rp1, a ginsenoside derivative, blocks lipopolysaccharide-induced interleukin-1beta production via suppression of the NF-kappaB pathway.

Abstract

Ginsenoside Rp1 (G-Rp1) is a ginseng saponin derivative with chemopreventive and anti-cancer activities. In this study, we examined the regulatory activity of G-Rp1 on the production of interleukin (IL)-1beta, a pro-inflammatory cytokine managing acute or chronic inflammatory diseases such as septic shock and rheumatoid arthritis, from lipopolysaccharide (LPS)-treated macrophage-like RAW264.7 cells. G-Rp1 dose-dependently inhibited IL-1beta production from LPS-treated RAW264.7 cells without altering cell viability. This compound suppressed both mRNA and protein levels of IL-1beta. In particular, this compound was found to down-regulate phosphorylation of the inhibitor of kappaB (IkappaB) kinase (IKK)/IkappaBalpha, and consequent activation of NF-kappaB, but not the activation of its upstream signaling enzymes such as mitogen-activated protein kinases (MAPK) and p85, a regulatory subunit of phosphoinositide 3-kinase (PI3K). Therefore, these results suggest that G-Rp1 may act as an inhibitor of IL-1beta production by inhibiting the NF-kappaB pathway.

• Authors: Byung Hun Kim, Yong Gyu Lee, Tae Yoon Park, Ho Bang Kim, Man Hee Rhee, Jae Youl Cho
• Journal: Planta medica (Planta Med) Vol. 75 Issue 4 Pg. 321-6 (Mar 2009) ISSN: 0032-0943 [Print] Germany
• PMID: 19145554 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ginsenosides
• Interleukin-1beta
• Lipopolysaccharides
• NF-kappa B
• RNA, Messenger
• ginsenoside Rp1
• lipopolysaccharide A

Topics

• Animals
• Cell Line
• Gene Expression Regulation (drug effects)
• Ginsenosides (chemistry, pharmacology)
• Interleukin-1beta (biosynthesis, drug effects)
• Lipopolysaccharides (toxicity)
• Macrophages (drug effects, metabolism)
• Molecular Structure
• NF-kappa B (drug effects, metabolism)
• RNA, Messenger (genetics, metabolism)