Abstract |
It was demonstrated that ascorbate- cobalt phthalocyanine complex produces a time-dependent nuclease effect on leukemia K-562 cells is. Catalase added to the incubation medium prevented or blocked fragmentation of cell DNA. The size of large-scale fragments formed during irradiation and exposure to the above system varied from 2200 to 30 kbp. The fragments induced by the system recombined slower than the fragments induced by g-irradiation in a dose adequate by the level of DNA damage. This effect observed previously in HEp-2 carcinoma cells exposed to the action of the B12b+C vitamin system can be explained by generation of H(2)O(2) inducing more severe damage to DNA structure than gamma-radiation due to site-specific Fenton reaction.
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Authors | A I Medvedev, V V Leschenko |
Journal | Bulletin of experimental biology and medicine
(Bull Exp Biol Med)
Vol. 145
Issue 5
Pg. 600-3
(May 2008)
ISSN: 0007-4888 [Print] United States |
PMID | 19145291
(Publication Type: Journal Article)
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Chemical References |
- DNA, Neoplasm
- Indoles
- Organometallic Compounds
- Reactive Oxygen Species
- cobalt phthalocyanine
- Ascorbic Acid
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Topics |
- Apoptosis
(drug effects, radiation effects)
- Ascorbic Acid
(pharmacology)
- DNA Fragmentation
(drug effects, radiation effects)
- DNA Repair
(drug effects, radiation effects)
- DNA, Neoplasm
(drug effects, metabolism, radiation effects)
- Gamma Rays
- Humans
- Indoles
(pharmacology)
- K562 Cells
- Organometallic Compounds
(pharmacology)
- Reactive Oxygen Species
(metabolism, radiation effects)
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