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Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry.

Abstract
Urinary-modified nucleosides have a potential role as cancer biomarkers for a number of malignant diseases. High performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify purine nucleosides purified from urine. Potential purine nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner, numerous modified purine nucleosides were identified in the urine samples from cancer patients including xanthine, adenosine, N1-methyladenosine, 5'-deoxy-5'-methylthioadenosine, 2-methyladenosine, N6-threonylcarbamoyladenosine, inosine, N1-methylinosine, guanosine, N1-methylguanosine, N7-methylguanine, N2-methylguanosine, N2,N2-dimethyguanosine, N2,N2,N7-trimethylguanosine. Furthermore, a number of novel purine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data including N3-methyladenosine, N7-methyladenine, 5'-dehydro-2'-deoxyinosine, N3-methylguanine, O6-methylguanosine, N1,N2,N7-trimethylguanosine, N1-methyl-N2-ethylguanosine and N7-methyl-N1-ethylguanosine.
AuthorsHua-Yu Li, Shao-Min Wang, Hong-Min Liu, Shan-Shan Bu, Juan Li, Dong Han, Ming-zhi Zhang, Guang-Yin Wu
JournalJournal of mass spectrometry : JMS (J Mass Spectrom) Vol. 44 Issue 5 Pg. 641-51 (May 2009) ISSN: 1096-9888 [Electronic] England
PMID19142897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright2009 John Wiley & Sons, Ltd.
Chemical References
  • Biomarkers, Tumor
  • Purine Nucleosides
Topics
  • Biomarkers, Tumor (urine)
  • Chromatography, High Pressure Liquid (methods)
  • Humans
  • Lymphoma (urine)
  • Purine Nucleosides (urine)
  • Tandem Mass Spectrometry (methods)

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