Tissue hemoglobin monitoring of progressive central hypovolemia in humans using broadband diffuse optical spectroscopy.

Abstract

We demonstrate noninvasive near-infrared diffuse optical spectroscopy (DOS) measurements of tissue hemoglobin contents that can track progressive reductions in central blood volume in human volunteers. Measurements of mean arterial blood pressure (MAP), heart rate (HR), stroke volume (SV), and cardiac output (Q) are obtained in ten healthy human subjects during baseline supine rest and exposure to progressive reductions of central blood volume produced by application of lower body negative pressure (LBNP). Simultaneous quantitative noninvasive measurements of tissue oxyhemoglobin (OHb), deoxyhemoglobin (RHb), total hemoglobin concentration (THb), and tissue hemoglobin oxygen saturation (S(t)O(2)) are performed throughout LBNP application using broadband DOS. As progressively increasing amounts of LBNP are applied, HR increases, and MAP, SV, and Q decrease (p<0.001). OHb, S(t)O(2), and THb decrease (p<0.001) in correlation with progressive increases in LBNP, while tissue RHb remained relatively constant (p=0.378). The average fractional changes from baseline values in DOS OHb (fOHb) correlate closely with independently measured changes in SV (r(2)=0.95) and Q (r(2)=0.98) during LBNP. Quantitative noninvasive broadband DOS measurements of tissue hemoglobin parameters of peripheral perfusion are capable of detecting progressive reductions in central blood volume, and appear to be sensitive markers of early hypoperfusion associated with hemorrhage as simulated by LBNP.

• Authors: Jangwoen Lee, Jae G Kim, Sari Mahon, Bruce J Tromberg, Kathy L Ryan, Victor A Convertino, Caroline A Rickards, Kathryn Osann, Matthew Brenner
• Journal: Journal of biomedical optics (J Biomed Opt) 2008 Nov-Dec Vol. 13 Issue 6 Pg. 064027 ISSN: 1083-3668 [Print] United States
• PMID: 19123673 (Publication Type: Evaluation Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Hemoglobins
• Oxygen

Topics

• Algorithms
• Diagnosis, Computer-Assisted (methods)
• Female
• Hemoglobins (analysis)
• Humans
• Hypovolemia (diagnosis, metabolism)
• Lower Body Negative Pressure (methods)
• Male
• Oxygen (analysis)
• Reproducibility of Results
• Sensitivity and Specificity
• Spectroscopy, Near-Infrared (methods)
• Young Adult