| Abstract | Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-? and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection. |
| Authors | Young Woo Han, Abi G Aleyas, Junu A George, Seon Ju Kim, Hye Kyung Kim, Hyun A Yoon, Dong Jin Yoo, Seong Ho Kang, Koanhoi Kim, Seong Kug Eo
(Affiliation: Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.)
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| Journal | Experimental & molecular medicine
(Exp Mol Med)
Vol. 40
Issue 6
Pg. 583-95
(Dec 31 2008)
ISSN: 1226-3613 Korea (South) |
| PMID | 19116444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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