A comparison was made between the levels of splenic and intestinal (Peyer's patches and thin intestinal epithelium) Ig production of C57BL/6 germ free and conventional C57BL/6, BALB/c, DBA/2 and C3H/He mice and the susceptibility to Mycobacterium avium
infection, evaluated by the number of bacterial colony-forming units (CFU) found in the liver and in the spleen of the animals. Mice received an i.p. injection of either 5 x 10(6), 10(7) or 10(8) bacteria, or were given the larger inoculum intragastrically. Alternatively, mice were treated with an i.p. injection of M. avium bacterial sonicates. A marked increase of splenic
IgA production, quantitatively associated with the size of the inoculum and thus with the degree of
infection, was observed in susceptible compared to relatively resistant mice. This increase was observed at an earlier time following
infection with the larger rather than with the smaller inocula. Consistent significant increases in splenic production of
IgG isotypes were only observed in the susceptible mice after
infection with the intermediate and larger inocula whereas a comparative increase of
IgM was only clearly observed after
infection with the larger inoculum. Intestinal Ig production remained unchanged, however, in both susceptible and relatively resistant mice after i.p.
infection. Also, all mice were resistant to M. avium
infection by the intragastric route and with this site of entry splenic and intestinal Ig production remained unchanged. Susceptibility to M. avium
infection was also quantitatively associated with increased levels of circulating specific anti-
bacterial antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)