Intravenous lipopolysaccharide-induced pulmonary maturation and structural changes in fetal sheep.

Abstract

BACKGROUND:

Antenatal pulmonary inflammation is associated with reduced risk for respiratory distress syndrome but with an increased risk for bronchopulmonary dysplasia (BPD) with impaired alveogenesis.

OBJECTIVE:

We hypothesized that fetal systemic inflammation induced by intravenous (IV) lipopolysaccharide (LPS) would affect lung development in utero.

STUDY DESIGN:

Twenty-one fetal sheep were instrumented (107 days gestational age). Control fetuses received saline (N = 12) and 9 in the study group received 100 ng of LPS IV 3 days after surgery. Animals were assessed for lung maturation and structure after 3 (N = 5) and 7 (N = 4) days.

RESULTS:

Interleukin-6 concentration increased in the bronchoalveolar lavage more than 40-fold 3 days after LPS IV. Processing of pro-surfactant protein (SP)-B to mature SP-B and increased SP-B concentrations were shown 7 days after LPS IV. Deposition of elastin fibers at sites of septation was disturbed within 3 days after LPS IV.

CONCLUSION:

Lung maturation and disturbed lung structure occurred after short-term exposure to fetal inflammation and suggests new targeted therapies for BPD.

• Authors: Boris W Kramer, Andreas Ladenburger, Steffen Kunzmann, Christian P Speer, Jasper V Been, J Freek van Iwaarden, Luc J I Zimmermann, Markus Gantert, Yves Garnier
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 200 Issue 2 Pg. 195.e1-10 (Feb 2009) ISSN: 1097-6868 [Electronic] United States
• PMID: 19110233 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipopolysaccharides
• Pulmonary Surfactant-Associated Protein B
• Elastin

Topics

• Animals
• Cell Proliferation
• Elastin (immunology)
• Female
• Fetal Diseases (immunology)
• Fetal Organ Maturity (immunology)
• Fetus
• Lipopolysaccharides (administration & dosage)
• Lung (growth & development, immunology)
• Lung Compliance
• Lung Diseases (immunology)
• Pulmonary Surfactant-Associated Protein B (immunology)
• Sheep