Decreased expression of 15-hydroxyprostaglandin dehydrogenase in gastric carcinomas.

Abstract

PURPOSE:

Gastric carcinoma tissues release high level of prostaglandin E2 (PGE2) when compared to non-neoplastic mucosa, and cyclooxygenase-2 (COX-2), which is the rate-limiting enzyme in prostaglandin (PG) biosynthesis, is often overexpressed in gastric carcinomas and during gastric carcinogenesis. However, little is known about the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), the key enzyme responsible for the biological inactivation of PG, in gastric carcinomas.

MATERIALS AND METHODS:

We investigated the expression of 15-PGDH in 28 cases of advanced gastric carcinomas by Western blot analysis and also the relation between its expression and the gene promoter methylation.

RESULTS:

15-PGDH expression was significantly decreased in gastric carcinomas compared to corresponding non-neoplastic tissues and inversely correlated with the expression of proliferating cell nuclear antigen in gastric carcinomas. However, there was no correlation between 15-PGDH expression and pathological findings such as nodal metastasis and vascular invasion. Promoter hypermethylation of 15-PGDH gene was not detected in carcinomas, with only a negligible expression of the enzyme.

CONCLUSION:

Our results suggested that 15-PGDH has tumor suppressor activity in gastric carcinomas.

• Authors: Tae Jung Jang, Ye Seob Ji, Ki Hoon Jung
• Journal: Yonsei medical journal (Yonsei Med J) Vol. 49 Issue 6 Pg. 917-22 (Dec 31 2008) ISSN: 0513-5796 [Print] Korea (South)
• PMID: 19108014 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• DNA, Neoplasm
• Hydroxyprostaglandin Dehydrogenases
• 15-hydroxyprostaglandin dehydrogenase

Topics

• Aged
• Base Sequence
• DNA Methylation
• DNA Primers (genetics)
• DNA, Neoplasm (genetics)
• Female
• Humans
• Hydroxyprostaglandin Dehydrogenases (genetics, metabolism)
• Male
• Middle Aged
• Promoter Regions, Genetic
• Stomach Neoplasms (enzymology, genetics)