Expression of centromere protein F (CENP-F) associated with higher FDG uptake on PET/CT, detected by cDNA microarray, predicts high-risk patients with primary breast cancer.

Abstract

BACKGROUND:

Higher standardized uptake value (SUV) detected by 18F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET/CT) correlates with proliferation of primary breast cancer. The purpose of this study is to identify specific molecules upregulated in primary breast cancers with a high SUV and to examine their clinical significance.

METHODS:

We compared mRNA expression profiles between 14 tumors with low SUVs and 24 tumors with high SUVs by cDNA microarray. We identified centromere protein F (CENP-F) and CDC6 were upregulated in tumors with high SUVs. RT-PCR and immunohistochemical analyses were performed to validate these data. Clinical implication of CENP-F and CDC6 was examined for 253 archival breast cancers by the tissue microarray.

RESULTS:

The relative ratios of CENP-F and CDC6 expression levels to beta-actin were confirmed to be significantly higher in high SUV tumors than in low SUV tumors (p = 0.027 and 0.025, respectively) by RT-PCR. In immunohistochemical analysis of 47 node-negative tumors, the CENP-F expression was significantly higher in the high SUV tumors (74%) than the low SUV tumors (45%) (p = 0.04), but membranous and cytoplasmic CDC6 expressions did not significantly differ between both groups (p = 0.9 each). By the tissue microarray, CENP-F (HR = 2.94) as well as tumor size (HR = 4.49), nodal positivity (HR = 4.1), and Ki67 (HR = 2.05) showed independent impact on the patients' prognosis.

CONCLUSION:

High CENP-F expression, correlated with high SUV, was the prognostic indicators of primary breast cancer. Tumoral SUV levels may serve as a pretherapeutic indicator of aggressiveness of breast cancer.

• Authors: Shigeto Ueda, Nobuo Kondoh, Hitoshi Tsuda, Souhei Yamamoto, Hideki Asakawa, Kazuhiko Fukatsu, Takayuki Kobayashi, Junji Yamamoto, Katsumi Tamura, Jiro Ishida, Yoshiyuki Abe, Mikio Yamamoto, Hidetaka Mochizuki
• Journal: BMC cancer (BMC Cancer) Vol. 8 Pg. 384 (Dec 22 2008) ISSN: 1471-2407 [Electronic] England
• PMID: 19102762 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• CDC6 protein, human
• Cell Cycle Proteins
• Chromosomal Proteins, Non-Histone
• Microfilament Proteins
• Nuclear Proteins
• centromere protein F
• Fluorodeoxyglucose F18

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Carcinoma, Ductal, Breast (diagnostic imaging, pathology)
• Cell Cycle Proteins (metabolism)
• Chromosomal Proteins, Non-Histone (metabolism)
• Data Interpretation, Statistical
• Female
• Fluorodeoxyglucose F18 (metabolism)
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• Immunohistochemistry
• Microfilament Proteins (metabolism)
• Middle Aged
• Nuclear Proteins (metabolism)
• Oligonucleotide Array Sequence Analysis (methods)
• Positron-Emission Tomography
• Predictive Value of Tests
• Prognosis
• Radiography
• Reproducibility of Results
• Reverse Transcriptase Polymerase Chain Reaction
• Statistics, Nonparametric