An anti-ulcerogenic flavonol diglucoside from Equisetum palustre L.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The aerial parts of Eqiusetum palustre L. is used to treat peptic ulcer in Karaman, Turkey.

AIM OF THE STUDY:

The present study was undertaken to evaluate the in vivo gastroprotective potential and structure elucidation of the active constituent(s) through bioassay-guided fractionation procedures by using ethanol (EtOH)-induced ulcerogenesis model in rats.

MATERIAL AND METHODS:

The 80% ethanol extract of the aerial parts was subjected to subsequent separation procedures such as solvent-solvent partition and column chromatography through bioassay-guided fractionation techniques to isolate the active anti-ulcer component(s) by using the EtOH-induced ulcer model in rats. The ulcer index was used to evaluate and to calculate the gastroprotection percentage.

RESULTS:

The subextracts, n-butanol and remaining H2O, of Equisetum palustre showed potent in vivo gastroprotective activity. Bioassay-guided fractionation has revealed that a flavonol diglucoside, was isolated as an active constituent from the n-BuOH subextract of Equisetum palustre. Structure elucidation by standard spectroscopic techniques (1H NMR, 13C NMR and DEPT, etc.) revealed that this compound as kaempferol-3-O-1''-beta-d-glucopyranosyl-3-O-1'''-beta-D-glucopyranoside, which provided remarkable protection at 250 and 500 mg/kg doses (69.5 and 98.5% ulcer inhibition, respectively) against ethanol-induced gastric lesions.

CONCLUSION:

Experimental data confirmed the ethnobotanical usage of Equisetum palustre, with scientific evidence, in Turkey. Additionally, kaempferol-3-O-1''-beta-D-glucopyranosyl-3-O-1'''-beta-D-glucopyranoside, a known compound, was defined as an anti-ulcerogenic compound in the present work for the first time.

• Authors: Ilhan Gurbuz, Erdem Yesilada, Shigeru Ito
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 121 Issue 3 Pg. 360-5 (Jan 30 2009) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 19100322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Ulcer Agents
• Kaempferols
• Plant Extracts
• kaempferol-3-O-1''-beta-d-glucopyranosyl-3-O-1'''-beta-D-glucopyranoside
• Misoprostol
• Ethanol

Topics

• Animals
• Anti-Ulcer Agents (therapeutic use)
• Disease Models, Animal
• Equisetum
• Ethanol
• Female
• Gastric Mucosa (drug effects)
• Kaempferols (chemistry, isolation & purification, therapeutic use)
• Male
• Misoprostol (therapeutic use)
• Phytotherapy
• Plant Components, Aerial
• Plant Extracts (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Stomach Ulcer (chemically induced, drug therapy)
• Turkey