Effect of allixin, a phytoalexin produced by garlic, on mutagenesis, DNA-binding and metabolism of aflatoxin B1.

Abstract

Allixin, a phytoalexin isolated from garlic, was examined for its effects on aflatoxin B1(AFB1)-induced mutagenesis using Salmonella typhimurium TA100 as the bacterial tester strain and rat liver S9 fraction as the metabolic activation system. The effects of allixin on the binding of [3H]AFB1 to calf thymus DNA and on the formation of metabolites of [3H]AFB1 were also determined. Allixin showed a dose-related inhibition of Histidine+ revertants induced by AFB1. Allixin at 75 micrograms/ml inhibited [3H]AFB1 binding to calf thymus DNA and reduced formation of AFB1-DNA adducts. In addition, allixin exhibited a concentration-dependent inhibition of the formation of organosoluble metabolites and the glutathione conjugates of [3H]AFB1. The data indicate that the effect of allixin on AFB1-induced mutagenesis and binding of metabolites to DNA may be mediated through an inhibition of microsomal P-450 enzymes. Allixin may thus be useful in the chemoprevention of cancer.

• Authors: T Yamasaki, R W Teel, B H Lau
• Journal: Cancer letters (Cancer Lett) Vol. 59 Issue 2 Pg. 89-94 (Aug 1991) ISSN: 0304-3835 [Print] Ireland
• PMID: 1909211 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aflatoxins
• Carcinogens
• Pyrones
• Tritium
• allixin
• DNA
• Aflatoxin B1

Topics

• Aflatoxin B1
• Aflatoxins (antagonists & inhibitors, metabolism)
• Animals
• Carcinogens (metabolism)
• DNA (metabolism)
• Garlic
• In Vitro Techniques
• Liver (metabolism)
• Male
• Molecular Structure
• Mutagenesis (drug effects)
• Mutagenicity Tests
• Plants, Medicinal
• Pyrones (chemistry, pharmacology)
• Rats
• Rats, Inbred Strains
• Salmonella typhimurium (genetics)
• Tritium