The protective effect of Egr-1 antisense oligodeoxyribonucleotide on myocardial injury induced by ischemia-reperfusion and hypoxia-reoxygenation.

Abstract

AIMS:

Our previous studies have shown that myocardial ischemia-reperfusion (I/R) injury is related closely with early growth response (Egr)-1 overexpression. The present study is to confirm thoroughly the effects of Egr-1 on the occurrance and development of myocardial I/R injury.

METHODS:

The Sprague-Dawley rat myocardial I/R model and cultured cardiomyocyte hypoxia-reoxygenation (H/R) model were established. The synthesized Egr-1 antisense oligodeoxyribonucleotide (AS-ODN) was transfected into myocardial tissues and cells. Hemodynamic parameters, myeloperoxidase (MPO), cardiac troponin I (cTnI), tumor necrosis factor-alpha (TNF-alpha), morphology, spontaneous beat and cell viability were measured to assess the degree of injury and inflammation of myocardial tissues and cells.

RESULTS:

In vivo, Egr-1 AS-ODN significantly attenuated injury and inflammation of myocardial tissues caused by I/R evidenced by the amelioration of hemodynamics and the reduction in MPO activity. In vitro, Egr-1 AS-ODN significantly relieved injury and inflammation of cultured cardiomyocyte caused by H/R evidenced by the improvement in morphology, structure and beat as well as the decrease in leakage of cTnI and release of TNF-alpha from cultured cardiomyocyte.

CONCLUSIONS:

These data suggest that Egr-1 plays a vital role in the pathogenesis of myocardial I/R injury and Egr-1 AS-ODN could protect the myocardium from I/R injury.

• Authors: Yanmei Zhang, Ganggang Shi, Jinhong Zheng, Yanqiu Lv, Ping Gao, Zhanqin Huang, Fenfei Gao, Yanqiong Zhou
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 22 Issue 5-6 Pg. 645-52 ( 2008) ISSN: 1421-9778 [Electronic] Germany
• PMID: 19088446 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2008 S. Karger AG, Basel.

Chemical References

• Culture Media
• Early Growth Response Protein 1
• Egr1 protein, rat
• Oligodeoxyribonucleotides, Antisense
• Protective Agents
• Troponin I
• Tumor Necrosis Factor-alpha
• Peroxidase
• Oxygen

Topics

• Animals
• Cell Hypoxia (drug effects)
• Cell Shape (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Culture Media
• Early Growth Response Protein 1 (metabolism)
• Hemodynamics (drug effects)
• Male
• Myocardial Reperfusion Injury (physiopathology, prevention & control)
• Myocardium (enzymology, pathology)
• Myocytes, Cardiac (drug effects, pathology)
• Oligodeoxyribonucleotides, Antisense (metabolism, pharmacology)
• Oxygen (pharmacology)
• Peroxidase (metabolism)
• Protective Agents (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Subcellular Fractions (metabolism)
• Troponin I (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)