Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS:
cPLA2-alpha is present in all prostate cancer cells lines, but increased in androgen-insensitive cells. Inhibition with small interfering RNA or Wyeth-1 results in significant reductions in prostate cancer cell numbers, as a result of reduced proliferation as well as increased apoptosis, and this was also associated with a reduction in cPLA2-alpha activity. Expression of cyclin D1 and phosphorylation of Akt were also observed to decrease. Wyeth-1 inhibited PC3 xenograft growth by approximately 33% and again, also reduced cyclin D1. Immunohistochemistry of human prostate tissue revealed that phosphorylated cPLA2-alpha is increased when hormone refractory is reached. CONCLUSIONS:
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Authors | Manish I Patel, Jaskirat Singh, Marzieh Niknami, Caroline Kurek, Mu Yao, Sasa Lu, Fiona Maclean, Nicholas J C King, Michael H Gelb, Kieran F Scott, Pamela J Russell, John Boulas, Qihan Dong |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 14
Issue 24
Pg. 8070-9
(Dec 15 2008)
ISSN: 1078-0432 [Print] United States |
PMID | 19088022
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCND1 protein, human
- Enzyme Inhibitors
- Cyclin D1
- Proto-Oncogene Proteins c-akt
- Group IV Phospholipases A2
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Topics |
- Apoptosis
- Cell Line, Tumor
- Cell Proliferation
- Cyclin D1
(analysis)
- Cytosol
(enzymology)
- Enzyme Inhibitors
(therapeutic use)
- Group IV Phospholipases A2
(antagonists & inhibitors)
- Humans
- Male
- Phosphorylation
- Prostatic Neoplasms
(drug therapy, enzymology, pathology)
- Proto-Oncogene Proteins c-akt
(metabolism)
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