| Abstract | We have examined the expression of two nuclear-acting oncogenes, c-fos and c-myc in the rat heart following administration of hormones implicated in the development of cardiac hypertrophy. A single injection of norepinephrine (2.5 micrograms/kg to 2.5 mg/kg) led to transient increases in the levels of both c-fos and c-myc mRNA. The response was sequential: elevated levels of c-fos mRNA were first observed 15 min after treatment and peaked at 1 h whilst c-myc mRNA levels increased 30 min after treatment and peaked at 2 h. The response of both cellular oncogenes to norepinephrine was reduced significantly by alpha blockade but beta blockade was less effective. Administration of triiodo-L-thyronine (0.25 mg/kg), a level known to promote cardiac hypertrophy, did not produce elevated levels of c-fos or c-myc mRNA. In an initial study, it was possible to demonstrate induction of c-fos and c-myc in rat hearts perfused in vitro with medium containing 2 x 10(-7) M norepinephrine. These results provide support for the notion that c-fos and c-myc expression may play a transducing role in the development of adrenergic-mediated, but not thyroid hormone-mediated cardiac hypertrophy. |
| Authors | R D Hannan, A K West
(Affiliation: Department of Biochemistry, University of Tasmania, Australia.)
|
| Journal | Basic research in cardiology
(Basic Res Cardiol)
1991 Mar-Apr
Vol. 86
Issue 2
Pg. 154-64
ISSN: 0300-8428 GERMANY |
| PMID | 1908674
(Publication Type: In Vitro, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-fos
- Proto-Oncogene Proteins c-myc
- RNA, Messenger
- Sympathomimetics
- Triiodothyronine
|
| Topics |
- Animals
- Blotting, Northern
- Gene Expression
- Heart
(drug effects)
- Male
- Myocardium
(metabolism)
- Perfusion
- Proto-Oncogene Proteins
(biosynthesis, genetics)
- Proto-Oncogene Proteins c-fos
- Proto-Oncogene Proteins c-myc
(biosynthesis, genetics)
- Proto-Oncogenes
- RNA, Messenger
(biosynthesis)
- Rats
- Rats, Inbred Strains
- Sympathomimetics
(pharmacology)
- Triiodothyronine
(pharmacology)
|
