Abstract | PURPOSE:
Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine shown to inhibit scar formation in fetal wound healing. The role of IL-10 in adult tendon healing and scar formation, however, remains unknown. The objective of this study is to investigate the effect of IL-10 overexpression on the properties of adult healing tendon using a well-established murine model of tendon injury and a lentiviral-mediated method of IL-10 overexpression. METHODS: A murine model of patellar tendon injury was used and animals divided into 3 groups. Mice received bilateral patellar tendon injections with a lentiviral vector containing an IL-10 transgene (n = 34) or no transgene (n = 34). Control mice (n = 34) received injections of sterile saline. All animals then were subjected to bilateral, central patellar tendon injuries 2 days after injection and were killed at 5, 10, 21, and 42 days after injury. IL-10 content was analyzed by immunohistochemistry (n = 4/group). Tendon healing was evaluated by histology (n = 4/group) and biomechanical analysis (n = 10/group). RESULTS: Overexpression of IL-10 in patellar tendon was confirmed after injection of the lentiviral vector. IL-10 immunostaining was increased at day 10 in the IL-10 group relative to that in controls. Histologically, there was no significant difference in angular deviation between groups at day 21, but a trend toward decreased angular deviation in controls relative to that in empty vector group mice was seen at day 42. Biomechanically, the IL-10 group showed significantly increased maximum stress at day 42 relative to that in controls. Percent relaxation showed a trend toward an increase at day 10 and a significant increase at day 42 in the IL-10 group relative to that in controls. CONCLUSIONS: This study demonstrates successful gene transfer of IL-10 into adult murine patellar tendon using a lentiviral vector. Although the effects of overexpression of IL-10 on adult tendon healing have not yet been fully elucidated, the current study may help to further clarify the mechanisms of tendon injury and repair.
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Authors | Eric T Ricchetti, Sudheer C Reddy, Heather L Ansorge, Miltiadis H Zgonis, Jonathan P Van Kleunen, Kenneth W Liechty, Louis J Soslowsky, Pedro K Beredjiklian |
Journal | The Journal of hand surgery
(J Hand Surg Am)
Vol. 33
Issue 10
Pg. 1843-52
(Dec 2008)
ISSN: 1531-6564 [Electronic] United States |
PMID | 19084188
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Disease Models, Animal
- Gene Transfer Techniques
- Genetic Therapy
- Genetic Vectors
- Interleukin-10
(physiology)
- Lentivirus
- Male
- Mice
- Patellar Ligament
(injuries)
- Tendon Injuries
(genetics, metabolism, therapy)
- Weight-Bearing
- Wound Healing
(physiology)
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