Abstract | BACKGROUND: METHODS: Specimens from pancreatic carcinoma and healthy parts of same pancreas were taken from 60 patients. Real time quantitative reverse transcription polymerase chain reaction estimated expression of HIF 1alpha, EPAS1, and VEGF mRNAs. Western blotting and immunohistochemical, streptavidin peroxidase method assessed expression of HIF 1alpha, EPAS1, and VEGF proteins. Microvessel density (MVD) was assessed. RESULTS: Highly significant increases in expression of EPAS1, VEGF, and MVD were found in pancreatic carcinoma tissue but not in normal pancreatic tissue: VEGF at mRNA and protein levels (t = 17.32, P = 0.0001; t = 98.41, P = 0.0001); EPAS1 protein level (t = 22.51, P = 0.0001). Expression of HIF 1alpha was similar in pancreatic carcinoma and normal pancreatic tissues at both mRNA and protein levels. Significant correlations were observed between EPAS1 and VEGF (r = 0.736, P = 0.0041), between VEGF and MVD (r = 0.858, P = 0.0001), and between EPAS1 and MVD (r = 0.641, P = 0.0003). No significant correlations were observed between HIF 1alpha and VEGF, or between HIF 1alpha and MVD. MVD and expression of EPAS1 and VEGF were significantly related with TNM staging, so was EPASI and VEGF with size of tumour. CONCLUSIONS:
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Authors | Dong-ming Zhu, De-chun Li, Zi-xiang Zhang, Xiao-yi Zhang |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 121
Issue 22
Pg. 2258-64
(Nov 20 2008)
ISSN: 2542-5641 [Electronic] China |
PMID | 19080330
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Basic Helix-Loop-Helix Transcription Factors
- Hypoxia-Inducible Factor 1, alpha Subunit
- Vascular Endothelial Growth Factor A
- endothelial PAS domain-containing protein 1
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Topics |
- Adult
- Aged
- Basic Helix-Loop-Helix Transcription Factors
(genetics, metabolism, physiology)
- Blotting, Western
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(genetics, metabolism)
- Immunohistochemistry
- Male
- Middle Aged
- Pancreatic Neoplasms
(genetics, metabolism, pathology)
- Reverse Transcriptase Polymerase Chain Reaction
- Vascular Endothelial Growth Factor A
(genetics, metabolism, physiology)
- Young Adult
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