Abstract | BACKGROUND: RESULTS: Prolonged upregulation of VGF mRNA and protein was observed in injured dorsal root ganglion neurons, central terminals and their target dorsal horn neurons. Intrathecal application of TLQP-62, the C-terminal active portion of VGF (5-50 nmol) to naïve rats caused a long-lasting mechanical and cold behavioral allodynia. Direct actions of 50 nM TLQP-62 upon dorsal horn neuron excitability was demonstrated in whole cell patch recordings in spinal cord slices and in receptive field analysis in intact, anesthetized rats where significant actions of VGF were upon spontaneous activity and cold evoked responses. CONCLUSION: VGF expression is therefore highly modulated in nociceptive pathways following peripheral nerve injury and can cause dorsal horn cell excitation and behavioral hypersensitivity in naïve animals. Together the results point to a novel and powerful role for VGF in neuropathic pain.
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Authors | Andrew Moss, Rachel Ingram, Stephanie Koch, Andria Theodorou, Lucie Low, Mark Baccei, Gareth J Hathway, Michael Costigan, Stephen R Salton, Maria Fitzgerald |
Journal | Molecular pain
(Mol Pain)
Vol. 4
Pg. 62
(Dec 10 2008)
ISSN: 1744-8069 [Electronic] United States |
PMID | 19077191
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neuropeptides
- RNA, Messenger
- VGF peptide
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Topics |
- Animals
- Cold Temperature
- Ganglia, Spinal
(metabolism)
- Models, Biological
- Neuralgia
(complications, metabolism)
- Neuropeptides
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Sensory Receptor Cells
(metabolism)
- Spinal Cord
(metabolism)
- Up-Regulation
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