Evaluation of staining methods for cytologic diagnosis of oral lesions.

Abstract

OBJECTIVE:

To compare the efficacy ofPapanicolaou, hematoxylin-eosin (H-E), Leishman and periodic acid-Schiff (PAS) staining for cytologic diagnosis of oral lesions.

STUDY DESIGN:

Patients from the Discipline of Stomatology, São José dos Campos Dental School, from the wards of Hosapital Heliópolis and from the dentistry outpatient clinic of the University Hospital, University of São Paulo Medical School, with the following diseases, were selected: erythematous candidiasis (n=9), pseudomembranous candidiasis (n=10), squamous cell carcinoma (n=19), herpes simplex (n=8), paracoccidioidomycosis (n=8) and pemphigus vulgaris (n=1).

RESULTS:

The different staining methods were compared regarding the quality of definition of cytoplasmic and nuclear morphologic characteristics and the identification of bacteria, fungi, inflammatory cells and secretions. Papanicolaou and H-E staining were considered better methods. In cases of fungal infections, PAS staining is useful and should be applied as a complementary method.

CONCLUSION:

Within the limitations of this study, it can be concluded that the cytologic diagnosis of oral lesions along with different staining methods is a useful tool for oral diagnosis.

• Authors: Janete Dias Almeida, Celina Faig Lima, Adriana Aigotti Haberbeck Brandão, Luiz Antonio Guimarães Cabral
• Journal: Acta cytologica (Acta Cytol) 2008 Nov-Dec Vol. 52 Issue 6 Pg. 697-701 ISSN: 0001-5547 [Print] Switzerland
• PMID: 19068674 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Eosine Yellowish-(YS)
• Hematoxylin

Topics

• Candidiasis (pathology)
• Carcinoma, Squamous Cell (pathology)
• Cytodiagnosis
• Diagnosis, Oral
• Eosine Yellowish-(YS)
• Hematoxylin
• Herpes Simplex (pathology)
• Humans
• Mouth Diseases (diagnosis)
• Paracoccidioidomycosis (pathology)
• Periodic Acid-Schiff Reaction
• Staining and Labeling (methods)
• Vaginal Smears