Expression and amplification of eIF-5A2 in human epithelial ovarian tumors and overexpression of EIF-5A2 is a new independent predictor of outcome in patients with ovarian carcinoma.

Abstract

OBJECTIVES:

Our previous study has suggested an oncogenic role of eIF-5A2 in ovarian tumorigenesis. Abnormalities of eIF-5A2 and its clinical/prognostic significance, however, in ovarian carcinoma are unclear.

METHODS:

In this study, we examined expression of EIF-5A2, using immunohistochemistry, in 30 normal ovaries, 30 ovarian cystadenomas, 30 borderline ovarian tumors and 110 ovarian carcinomas. The amplification status of eIF-5A2 in each ovarian carcinoma was assessed by fluorescence in situ hybridization.

RESULTS:

Overexpression of EIF-5A2 was detected in none of the normal ovaries, 7% cystadenomas, 30% borderline tumors, and 53% invasive ovarian carcinomas, respectively. Amplification of eIF-5A2 was detected in 16% of informative ovarian carcinomas. In ovarian carcinomas, significant positive associations were found between overexpression of EIF-5A2 and the tumors ascending grade, later pT/pN and FIGO stages, as well as increased positive rate of Ki-67 (p<0.05). In univariate survival analysis of the ovarian carcinoma cohorts, a significant association of overexpression of EIF-5A2 with shortened patient survival (mean 39.0 months vs 69.5 months, p<0.001) was demonstrated. Importantly, EIF-5A2 expression provided significant independent prognostic parameters in multivariate analysis (p=0.043).

CONCLUSIONS:

These findings suggest that increased expression of EIF-5A2 in ovarian carcinoma may represent an acquired malignant phenotypic feature of tumor cells, and the overexpression of EIF-5A2, as detected by immunohistochemistry, is an independent molecular marker for shortened survival time of patients with ovarian carcinoma.

• Authors: Guo-Fen Yang, Dan Xie, Ji-Hong Liu, Jun-Hang Luo, Li-Juan Li, Wen-Feng Hua, Hong-Mei Wu, Hsiang-Fu Kung, Yi-Xin Zeng, Xin-Yuan Guan
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 112 Issue 2 Pg. 314-8 (Feb 2009) ISSN: 1095-6859 [Electronic] United States
• PMID: 19054548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Ki-67 Antigen
• Peptide Initiation Factors
• eIF-5A2

Topics

• Adult
• Aged
• Biomarkers, Tumor (biosynthesis, genetics)
• Cystadenoma (genetics, metabolism, pathology)
• Female
• Gene Amplification
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Ki-67 Antigen (biosynthesis)
• Middle Aged
• Ovarian Neoplasms (genetics, metabolism, pathology)
• Paraffin Embedding
• Peptide Initiation Factors (biosynthesis, genetics)
• Prognosis
• Survival Analysis
• Young Adult