Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils.

Abstract

The present study evaluated the anti-inflammatory activity of amburoside A (a phenol glucoside) and isokaempferide (a flavonol) isolated from the trunk bark of Amburana cearensis, a medicinal plant used in northeast Brazil for the treatment of asthma. Animals (male Wistar rats or Swiss mice) pre-treated with amburoside A (25 and 50 mg/kg) or isokaempferide (12.5, 25 and 50 mg/kg), orally or intraperitoneally, showed a significant inhibition of the paw oedema induced by carrageenan (1%), prostaglandin E(2) (30 nmol/paw), histamine (200 microg/paw) or serotonin (200 microg/paw). Histological and morphometric evaluations of the rat paw oedema induced by carrageenan showed that amburoside A and isokaempferide also inhibited the accumulation of inflammatory cells. Amburoside A reduced significantly the paw oedema and the increase in vascular permeability induced by dextran, as related to the control group. Similar results were observed with the isokaempferide pre-treatment. Furthermore, amburoside A or isokaempferide inhibited both leucocyte and neutrophil migrations, in mouse peritoneal cavity, after the carrageenan injection. The polyphenols were not cytotoxic and blocked N-formyl-methyl-leucyl-phenylalanine-induced myeloperoxidase release and activity in human neutrophils. In addition, amburoside A and isokaempferide at 50 and 100 microg/ml concentrations reduced significantly the lipopolysaccharide-mediated increase in tumour necrosis factor-alpha (TNF-alpha) levels. These results provide, for the first time, evidence to support the anti-inflammatory activity of amburoside A and isokaempferide that seems to be related to an inhibition of inflammatory mediators, such as TNF-alpha, as well as histamine, serotonin and prostaglandin E(2), besides leucocyte infiltration in a dose- or concentration-dependent manner. These anti-inflammatory effects can be explained, at least in part, by the ability of these compounds to reduce neutrophil degranulation, myeloperoxidase activity, mediators as well as TNF-alpha secretion.

• Authors: Luzia Kalyne Almeida Moreira Leal, Kirley Marques Canuto, Kassiane Cristine da Silva Costa, Hélio Vitoriano Nobre-Júnior, Silvânia Mendes Vasconcelos, Edilberto Rocha Silveira, Márcia Valéria Pitombeira Ferreira, Juvênia Bezerra Fontenele, Geane Matos Andrade, Glauce Socorro de Barros Viana
• Journal: Basic & clinical pharmacology & toxicology (Basic Clin Pharmacol Toxicol) Vol. 104 Issue 3 Pg. 198-205 (Mar 2009) ISSN: 1742-7843 [Electronic] England
• PMID: 19053991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Flavonoids
• Glucosides
• Inflammation Mediators
• Phenols
• Plant Extracts
• Polyphenols
• Tumor Necrosis Factor-alpha
• amburoside A
• isokaempferide
• Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, isolation & purification, pharmacology)
• Brazil
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Fabaceae (chemistry)
• Flavonoids (administration & dosage, isolation & purification, pharmacology, toxicity)
• Glucosides (administration & dosage, isolation & purification, pharmacology)
• Humans
• Inflammation (drug therapy, physiopathology)
• Inflammation Mediators (metabolism)
• Male
• Medicine, Traditional
• Mice
• Neutrophils (drug effects, metabolism)
• Peroxidase (drug effects, metabolism)
• Phenols (isolation & purification, pharmacology, toxicity)
• Plant Bark
• Plant Extracts (administration & dosage, pharmacology)
• Polyphenols
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (drug effects, metabolism)