Abstract |
Growth of maize (Zea mays L.) callus-culture cells was inhibited using dichlobenil (2,6 dichlorobenzonitrile, DCB) concentrations > or =1 microM; I (50) value for the effect on inhibited fresh weight gain was 1.5 microM. By increasing the DCB concentration in the culture medium, DCB-habituated cells became 13 times more tolerant of the inhibitor (I (50): 20 microM). In comparison with non-habituated calluses, DCB-habituated calluses grew slower, were less friable and were formed by irregularly shaped cells surrounded by a thicker cell wall. By using an extensive array of techniques, changes in type II cell wall composition and structure associated with DCB habituation were studied. Walls from DCB-habituated cells showed a reduction of up to 75% in cellulose content, which was compensated for by a net increase in arabinoxylan content. Arabinoxylans also showed a reduction in their extractability and a marked increase in their relative molecular mass. DCB habituation also involved a shift from ferulate to coumarate-rich cells walls, and enrichment in cell wall esterified hydroxycinnamates and dehydroferulates. The content of polymers such as mixed- glucan, xyloglucan, mannans, pectins or proteins did not vary or was reduced. These results prove that the architecture of type II cell walls is able to compensate for deficiencies in cellulose content with a more extensive and phenolic cross-linked network of arabinoxylans, without necessitating beta-glucan or other polymer enhancement. As a consequence of this modified architecture, walls from DCB-habituated cells showed a reduction in their swelling capacity and an increase both in pore size and in resistance to polysaccharide hydrolytic enzymes.
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Authors | Hugo Mélida, Penélope García-Angulo, Ana Alonso-Simón, Antonio Encina, Jesús Alvarez, José Luis Acebes |
Journal | Planta
(Planta)
Vol. 229
Issue 3
Pg. 617-31
(Feb 2009)
ISSN: 0032-0935 [Print] Germany |
PMID | 19048286
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amino Acids
- Culture Media
- Nitriles
- Phenols
- Plant Proteins
- Xylans
- Cellulose
- arabinoxylan
- dichlobanil
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Topics |
- Amino Acids
(analysis)
- Carbohydrate Metabolism
- Cell Culture Techniques
- Cell Fractionation
- Cell Wall
(chemistry, drug effects, metabolism)
- Cellulose
(chemistry, metabolism)
- Chromatography, Gel
- Culture Media
- Nitriles
(pharmacology)
- Phenols
(analysis)
- Plant Proteins
(metabolism)
- Spectroscopy, Fourier Transform Infrared
- Xylans
(chemistry, metabolism)
- Zea mays
(cytology, drug effects, ultrastructure)
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