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Novel type II cell wall architecture in dichlobenil-habituated maize calluses.

Abstract
Growth of maize (Zea mays L.) callus-culture cells was inhibited using dichlobenil (2,6 dichlorobenzonitrile, DCB) concentrations > or =1 microM; I (50) value for the effect on inhibited fresh weight gain was 1.5 microM. By increasing the DCB concentration in the culture medium, DCB-habituated cells became 13 times more tolerant of the inhibitor (I (50): 20 microM). In comparison with non-habituated calluses, DCB-habituated calluses grew slower, were less friable and were formed by irregularly shaped cells surrounded by a thicker cell wall. By using an extensive array of techniques, changes in type II cell wall composition and structure associated with DCB habituation were studied. Walls from DCB-habituated cells showed a reduction of up to 75% in cellulose content, which was compensated for by a net increase in arabinoxylan content. Arabinoxylans also showed a reduction in their extractability and a marked increase in their relative molecular mass. DCB habituation also involved a shift from ferulate to coumarate-rich cells walls, and enrichment in cell wall esterified hydroxycinnamates and dehydroferulates. The content of polymers such as mixed-glucan, xyloglucan, mannans, pectins or proteins did not vary or was reduced. These results prove that the architecture of type II cell walls is able to compensate for deficiencies in cellulose content with a more extensive and phenolic cross-linked network of arabinoxylans, without necessitating beta-glucan or other polymer enhancement. As a consequence of this modified architecture, walls from DCB-habituated cells showed a reduction in their swelling capacity and an increase both in pore size and in resistance to polysaccharide hydrolytic enzymes.
AuthorsHugo Mélida, Penélope García-Angulo, Ana Alonso-Simón, Antonio Encina, Jesús Alvarez, José Luis Acebes
JournalPlanta (Planta) Vol. 229 Issue 3 Pg. 617-31 (Feb 2009) ISSN: 0032-0935 [Print] Germany
PMID19048286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amino Acids
  • Culture Media
  • Nitriles
  • Phenols
  • Plant Proteins
  • Xylans
  • Cellulose
  • arabinoxylan
  • dichlobanil
Topics
  • Amino Acids (analysis)
  • Carbohydrate Metabolism
  • Cell Culture Techniques
  • Cell Fractionation
  • Cell Wall (chemistry, drug effects, metabolism)
  • Cellulose (chemistry, metabolism)
  • Chromatography, Gel
  • Culture Media
  • Nitriles (pharmacology)
  • Phenols (analysis)
  • Plant Proteins (metabolism)
  • Spectroscopy, Fourier Transform Infrared
  • Xylans (chemistry, metabolism)
  • Zea mays (cytology, drug effects, ultrastructure)

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