Anti-inflammatory, analgesic and wound healing activities of the leaves of Memecylon edule Roxb.

Abstract

AIM OF THE STUDY:

To determine the anti-inflammatory, analgesic and antioxidant activities of the leaves of Memecylon edule Roxb. used traditionally in Thailand.

MATERIALS AND METHODS:

Hexane, (Hex), ethyl acetate (EtOAc), methanol (MeOH) and 50% methanol (MeOH50) fractions of the dry leaves were tested in vitro for their interleukin-10 production; the most active fraction was further studied in vivo for its anti-inflammatory and analgesic activities using the ethylphenylpropiolate (EPP)-induced mouse ear edema and the writhing test with mice. All fractions except Hex were tested for their radical scavenging activity towards 1'-diphenyl-2-picrylhydrazyl radical (DPPH).

RESULTS:

The EtOAc showed the highest stimulation for interleukin-10 production. In the EPP test, this fraction was significantly active 30 min after topical application at all doses used (0.5, 1.0, 2.0mg/ear); after 4h and at 1.0mg/ear EtOAc was slightly less active (inhibition 47.8%) than the reference, indomethacin, at the same dose (62.4%). At 200mg/kg orally, the EtOAc caused a significant inhibition of the writhing response by 56.6% which was like indomethacin at 10mg/kg. EtOAc, MeOH and MeOH50 exhibited radical scavenging activity. The order of IC(50) values was: ascorbic acid (9.1 microg/mL)>trolox (11.6 microg/mL)>MeOH (46.9 microg/mL)>MeOH50 (152.1 microg/mL)>EtOAc (1742.2 microg/mL).

CONCLUSION:

The results provide support for the traditional use of Memecylon edule leaves in relieving inflammation and pain.

• Authors: Somsak Nualkaew, Kwanchai Rattanamanee, Suchitra Thongpraditchote, Yuwadee Wongkrajang, Adolf Nahrstedt
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 121 Issue 2 Pg. 278-81 (Jan 21 2009) ISSN: 0378-8741 [Print] Ireland
• PMID: 19041700 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics
• Anti-Inflammatory Agents
• Antioxidants
• Plant Extracts
• Interleukin-10
• Indomethacin

Topics

• Analgesics (administration & dosage, isolation & purification, pharmacology)
• Animals
• Anti-Inflammatory Agents (administration & dosage, isolation & purification, pharmacology)
• Antioxidants (administration & dosage, isolation & purification, pharmacology)
• Cell Line
• Dose-Response Relationship, Drug
• Female
• Indomethacin (pharmacology)
• Inflammation (drug therapy, physiopathology)
• Interleukin-10 (metabolism)
• Macrophages (drug effects, metabolism)
• Medicine, East Asian Traditional
• Melastomataceae (chemistry)
• Mice
• Plant Extracts (administration & dosage, pharmacology)
• Plant Leaves
• Thailand
• Wound Healing (drug effects)