[Change of CCR7 and CD45RA after blocking of Kv1.3 potassium channel of CD4+ T lymphocytes in multiple sclerosis].

Abstract

OBJECTIVE:

To investigate the changes of CCR7 and CD45RA expression after blocking of the potassium channel Kv1.3 in myelin specific CD4 T lymphocytes and the relation thereof with multiple sclerosis(MS).

METHODS:

Peripheral blood mononuclear cells were isolated from 15 activated MS patients, 15 INF-beta-1b treated MS patients, and 15 normal controls, CD4+ T lymphocytes were isolated using positive selection method with anti-CD4-coated magnetic beads. To establish culturing MBP special CD4+ T lymphocyte lines, the different groups of T cell were labeled with CD3, CD4, CCR7, and CD45RA fluorescence-antibody or homotype controls and analyzed by four-color flow cytometer.

RESULTS:

The most part of phenotype in the activated MS patients was CD4+ CCR7- CD45RA- T cells and the percentage was increased after myelin antigen stimulation (P < 0.05), whereas the percentage of CCR7+ CD45RA+ T cells was decreased (P < 0.05). SHK greatly inhibited CCR7- CD45RA- in activated MS (P < 0.05). CCR7-CD45RA- and CCR7+ CD45RA- were in correlation with expanded disability status scale (EDSS) score (r = 0.73, r = 0.705, P < 0.05) in the peripheral blood of activated MS.

CONCLUSION:

There is a strong correlation between T(EM) phenotype and severity of MS, which may suggest Tem phenotype as the marker to estimate the state of illness. Kv1.3 potassium channel may be the new target in treatment of MS.

• Authors: Xiao-tun Ren, Wei-ping Wu, Quan-gang Xu, De-hui Huang
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 88 Issue 27 Pg. 1896-9 (Jul 15 2008) ISSN: 0376-2491 [Print] China
• PMID: 19040002 (Publication Type: Journal Article)

Chemical References

• CCR7 protein, human
• Calcium Channel Blockers
• Cnidarian Venoms
• Kv1.3 Potassium Channel
• Receptors, CCR7
• Leukocyte Common Antigens

Topics

• Adult
• CD4-Positive T-Lymphocytes (cytology, drug effects, metabolism)
• Calcium Channel Blockers (pharmacology)
• Cells, Cultured
• Cnidarian Venoms (pharmacology)
• Female
• Flow Cytometry
• Humans
• Kv1.3 Potassium Channel (antagonists & inhibitors)
• Leukocyte Common Antigens (biosynthesis)
• Male
• Middle Aged
• Multiple Sclerosis (blood)
• Receptors, CCR7 (biosynthesis)
• Young Adult