[Quantitative detection of hepatitis B virus covalently closed circular DNA in sera of chronic hepatitis B patients with a newly established assay].

Abstract

OBJECTIVE:

To quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.

METHODS:

Primers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA). The products were amplified by real-time PCR with primers spanning.

RESULTS:

The detection rate of serum HBV cccDNA was found to correlate directly with serum HBV DNA loading. HBeAg positive chronic hepatitis B patients had higher serum HBV cccDNA levels than HBeAg negative chronic hepatitis B patients.

CONCLUSION:

The method is good because of the high specificity. It can be used for detection of HBV cccDNA. DNA;

• Authors: Yan-Wei Zhong, Zhao-Ling Liang, Xiao-Qiang Ren, Xiao-Dong Li, Zhi-Hui Xu, Le Li, Dong-Ping Xu
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 22 Issue 3 Pg. 225-7 (Jun 2008) ISSN: 1003-9279 [Print] China
• PMID: 19031711 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• DNA, Circular
• DNA, Viral

Topics

• Adolescent
• Adult
• DNA Primers (genetics)
• DNA, Circular (blood, genetics)
• DNA, Viral (blood, genetics)
• Female
• Hepatitis B virus (genetics)
• Hepatitis B, Chronic (virology)
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction (methods)
• Young Adult