HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The alcelaphine herpesvirus-1 ORF 57 encodes a nuclear shuttling protein.

Abstract
Alcelaphine herpesvirus-1 (AlHV-1) is a gamma(2) rhadinovirus associated with Malignant Catarrhal Fever (MCF) in cattle. ORF 57 is well conserved among gammaherpesviruses and it has been shown that the ORF 57 gene products of Herpesvirus Saimiri (HVS), Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) play an important role in regulating viral gene expression. The AlHV-1 ORF 57 gene product has not been characterized. In the accompanying paper we have demonstrated that AlHV-1 ORF 57 encodes an immediate early protein that acts as a regulator of gene expression. The ORF 57 gene product has an up-regulatory effect only on another immediate early gene product encoded by ORF 50. Here we show that the ORF 57 gene product is a nuclear protein. When ORF 57 was fused to the gene encoding Enhanced Green Fluorescent Protein (EGFP), the fusion protein exhibited a punctate nuclear distribution that co-localized with the nucleolar phosphoprotein C23. The nuclear localisation signal of ORF 57 gene product was located at the N-terminus. The ORF 57 gene product travels from nucleus to the cytoplasm, where it accumulates during Actinomycin D treatment. The domain involved in nuclear shuttling was also localised at the N-terminal region of the protein. Thus in common with homologues in other herpesviruses the AlHV-1 ORF 57 gene product is a nuclear cytoplasmic shuttling protein which may play a role in export of viral mRNAs from the nucleus of infected cells.
AuthorsT Leenadevi, R G Dalziel
JournalVeterinary research communications (Vet Res Commun) Vol. 33 Issue 5 Pg. 409-19 (Jun 2009) ISSN: 1573-7446 [Electronic] Switzerland
PMID19031003 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Nuclear Localization Signals
  • Viral Proteins
Topics
  • Animals
  • Cell Line
  • Cricetinae
  • Gene Expression Regulation, Viral (physiology)
  • Herpesviridae (genetics, metabolism)
  • Nuclear Localization Signals
  • Open Reading Frames (physiology)
  • Protein Transport (physiology)
  • Viral Proteins (physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: