This study was carried out to determine the prevalence of Theileria annulata, Theileria mutans, Babesia bigemina, and Anaplasma marginale
antibodies among cattle in the Sudan. A total of 600 serum samples were collected from indigenous (zebu) and crossbred cattle (zebu x Friesian) of both sex and different age groups. Indirect
enzyme-linked
immunosorbent assay was used to assess
antibodies against
tick-borne diseases in apparently healthy cattle. The overall prevalence rates of T. annulata, T. mutans, B. bigemina, and A. marginale
antibodies were found to be 30.8%, 6.1%, 10.7%, and 38.9%, respectively. The highest seroprevalence of T. annulata was reported in Atbara and El Damer, Northern Sudan. There were no significant associations for the seroprevalence of all
tick-borne diseases reported among different age groups. Although there were no significant differences between the two breeds of cattle examined for T. annulata, T. mutans, and B. bigemina
antibodies, there was a significant difference for prevalence of
antibodies against A. marginale, with highest percentages of
antibodies in indigenous cattle. Six different combinations of
mixed infection were detected. This is the first report in which
antibodies against A. marginale among cattle in Northern Sudan is reported. The findings imply that
antibodies to
tick-borne infections are widely distributed in the region. The need for further investigations using more advanced techniques is recommended.