Interference in clinical chemistry assays by the hemoglobin-based oxygen carrier, Hemospan.

Abstract

OBJECTIVE:

To evaluate interference by the hemoglobin-based oxygen carrier Hemospan on clinical laboratory assays.

DESIGN AND METHODS:

Interfering Hemospan concentrations were determined for general chemistry and cardiac marker analytes in pooled serum and the corresponding hemolysis index was calculated.

RESULTS:

Hemospan did not interfere with 20 of 35 analytes. Hemospan produced a negative interference in serum creatinine, amylase, alkaline phosphatase, uric acid, and GGT assays and a positive interference in serum phosphate, LDH, iron, triglycerides, total protein, AST, cholesterol, magnesium, and albumin assays, and appeared to positively bias the serum cardiac troponin I (cTnI) assay only when cTnI is present in the sample.

CONCLUSIONS:

We present a report of assays affected by Hemospan and the threshold concentrations for interference. This study highlights the importance of interference studies in understanding the effects of hemoglobin-based oxygen carriers on results reported by the clinical laboratory.

• Authors: Scott J Cameron, Gregory Gerhardt, Michael Engelstad, Mark A Young, Edward J Norris, Lori J Sokoll
• Journal: Clinical biochemistry (Clin Biochem) Vol. 42 Issue 3 Pg. 221-4 (Feb 2009) ISSN: 1873-2933 [Electronic] United States
• PMID: 19028481 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Substitutes
• Hemoglobins
• Maleimides
• maleimide-polyethylene glycol-modified hemoglobin, MP4
• Polyethylene Glycols

Topics

• Blood Chemical Analysis (standards)
• Blood Substitutes
• Chemistry, Clinical (methods, standards)
• Diagnostic Errors
• Hemoglobins
• Humans
• Maleimides
• Polyethylene Glycols
• Reproducibility of Results