Abstract |
Two full-length genes encoding different acetylcholinesterases ( AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.
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Authors | Xiaojing Jiang, Mingjing Qu, Ian Denholm, Jichao Fang, Weihua Jiang, Zhaojun Han |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 378
Issue 2
Pg. 269-72
(Jan 09 2009)
ISSN: 1090-2104 [Electronic] United States |
PMID | 19028456
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Insecticides
- Organothiophosphates
- Triazoles
- triazophos
- Acetylcholinesterase
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Topics |
- Acetylcholinesterase
(genetics)
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Genes, Insect
- Insecticide Resistance
(genetics)
- Insecticides
(pharmacology)
- Lepidoptera
(drug effects, enzymology, genetics)
- Molecular Sequence Data
- Mutation
- Organothiophosphates
(pharmacology)
- Oryza
(parasitology)
- Plant Diseases
(parasitology)
- Polymorphism, Restriction Fragment Length
- Sequence Analysis, DNA
(methods)
- Sequence Analysis, Protein
- Triazoles
(pharmacology)
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