HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae).

Abstract
Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.
AuthorsXiaojing Jiang, Mingjing Qu, Ian Denholm, Jichao Fang, Weihua Jiang, Zhaojun Han
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 378 Issue 2 Pg. 269-72 (Jan 09 2009) ISSN: 1090-2104 [Electronic] United States
PMID19028456 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Insecticides
  • Organothiophosphates
  • Triazoles
  • triazophos
  • Acetylcholinesterase
Topics
  • Acetylcholinesterase (genetics)
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Genes, Insect
  • Insecticide Resistance (genetics)
  • Insecticides (pharmacology)
  • Lepidoptera (drug effects, enzymology, genetics)
  • Molecular Sequence Data
  • Mutation
  • Organothiophosphates (pharmacology)
  • Oryza (parasitology)
  • Plant Diseases (parasitology)
  • Polymorphism, Restriction Fragment Length
  • Sequence Analysis, DNA (methods)
  • Sequence Analysis, Protein
  • Triazoles (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: