Abstract | OBJECTIVE: METHODS:
Double stranded RNA (dsRNA) targeting the livin gene was chemically synthesized in vitro and transfected into human breast cancer cells of the line ZR-75-30 mediated by lipofectamine 2000. The transfection efficiency was observed by fluorescence confocal microscopy. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of livin at mRNA and protein levels. ZR-75-30 cells transfected with dsRNA targeting livin for 24 h were treated with 50 microg/ml epirubicin for 12 h, flow cytometry was used to detect the apoptosis rate of the cells. RESULTS: The livin mRNA expression rates of the livin- siRNA transfected group was (29.68 +/- 2.7)%, with an inhibitory rate of 53.66%, significantly lower than those of the negative control and blank control groups [(52.01 +/- 2.9)% and (51.95 +/- 3.1)% respectively, both P < 0.01]. The livin protein expression rate of the livin- siRNA group was (27.80 +/- 2.1)%, significantly lower than those of the negative control siRNA group and blank control group [(53.80 +/- 3.0)% and (55.12 +/- 2.8)% respectively, both P < 0.01]. 36 h after the treatment of siRNA against livin combined with epirubicin the apoptosis rate was (15.18 +/- 0.05)%, significantly higher than those of the negative control group and blank control group [(2.78 +/- 0.08)% and (2.65 +/- 0.12)% respectively, both P < 0.01]. CONCLUSIONS:
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Authors | Chun-yan Liang, Ping Ma, Xin-li Liu |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 88
Issue 24
Pg. 1703-6
(Jun 24 2008)
ISSN: 0376-2491 [Print] China |
PMID | 19024543
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- BIRC7 protein, human
- Inhibitor of Apoptosis Proteins
- Neoplasm Proteins
- RNA, Small Interfering
- Epirubicin
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Topics |
- Adaptor Proteins, Signal Transducing
(genetics, metabolism)
- Apoptosis
(drug effects, genetics)
- Blotting, Western
- Breast Neoplasms
(genetics, pathology)
- Cell Line, Tumor
- Epirubicin
(pharmacology)
- Female
- Flow Cytometry
- Gene Silencing
- Humans
- Inhibitor of Apoptosis Proteins
(genetics, metabolism)
- Microscopy, Confocal
- Neoplasm Proteins
(genetics, metabolism)
- RNA, Small Interfering
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
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