Production of polyclonal and monoclonal antibodies for specific detection of nitrosation-proficient denitrifying bacteria in biological fluids.

Abstract

Polyclonal and monoclonal antibodies were raised against the nitrosating enzyme previously isolated and purified from a denitrifying bacteria Pseudomonas aeruginosa. Using the polyclonal antibodies, a preliminary ELISA test was set up which allowed the detection of the nitrosating enzyme in 2 ml urine samples with a minimal total bacteria count of greater than or equal to 10(5) cells/ml. The use of such a rapid immunological screening test in clinical settings should ascertain whether individual subjects at higher risk for cancer of the stomach or bladder harbour more nitrosation-proficient microorganisms in their microflora. The availability of monoclonal antibodies provides a tool for studying the mechanisms of bacteria-mediated nitrosamine formation from precursor amines.

• Authors: N D Venezia, S Calmels, H Bartsch
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 176 Issue 1 Pg. 262-8 (Apr 15 1991) ISSN: 0006-291X [Print] United States
• PMID: 1902104 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• Antibodies, Monoclonal
• Oxidoreductases
• nitrosating enzyme

Topics

• Animals
• Antibodies
• Antibodies, Monoclonal
• Bacteria (enzymology, isolation & purification)
• Body Fluids (microbiology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunoblotting
• Mice
• Mice, Inbred BALB C (immunology)
• Oxidoreductases (analysis, immunology)
• Pseudomonas aeruginosa (enzymology, isolation & purification)
• Rabbits (immunology)
• Urine (microbiology)