Abstract |
Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.
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Authors | Ying Gu, Zhiping Deng, Alexander R Paredez, Seth DeBolt, Zhi-Yong Wang, Chris Somerville |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 105
Issue 46
Pg. 18064-9
(Nov 18 2008)
ISSN: 1091-6490 [Electronic] United States |
PMID | 19004800
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Arabidopsis Proteins
- Dinitrobenzenes
- Molecular Chaperones
- Multiprotein Complexes
- Mutant Proteins
- Sulfanilamides
- Tubulin
- prefoldin
- prefoldin 6 protein, Arabidopsis
- oryzalin
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Topics |
- Arabidopsis
(cytology, drug effects, metabolism)
- Arabidopsis Proteins
(chemistry, metabolism)
- Cell Division
(drug effects)
- Cloning, Molecular
- Dinitrobenzenes
(pharmacology)
- Electrophoresis, Gel, Two-Dimensional
- Hypocotyl
(cytology, drug effects, metabolism)
- Microtubules
(drug effects, metabolism)
- Molecular Chaperones
(chemistry, metabolism)
- Multiprotein Complexes
(metabolism)
- Mutant Proteins
(isolation & purification, metabolism)
- Mutation
(genetics)
- Phenotype
- Plant Roots
(anatomy & histology, cytology, drug effects)
- Protein Folding
(drug effects)
- Proteomics
- Seedlings
(cytology, drug effects, growth & development, metabolism)
- Sulfanilamides
(pharmacology)
- Tubulin
(metabolism)
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