Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells.

Abstract

We report here that a microbial extracellular glycolipid,mannosylerythritol lipid (MEL), induces the outgrowth ofneurites from and enhances the activity of acetylcholinesterase(AChE) in PC12 pheochromocytoma cells. Furthermore, treatment ofPC12 cells with MEL increased levels of galactosylceramide(Galbeta1-1'Cer; GalCer). Exposure of PC12 cells to exogenous GalCer caused the dose-dependent outgrowth ofneurites. By contrast, treatment of PC12 cells with nerve growthfactor (NGF) did not increase the level of GalCer in the cells. The neurite-related morphological changes induced by GalCerdifferend from those induced by NGF, indicating differencesbetween the signal transduction pathways triggered by NGF and by GalCer.

• Authors: M Shibahara, X Zhao, Y Wakamatsu, N Nomura, T Nakahara, C Jin, H Nagaso, T Murata, K K Yokoyama
• Journal: Cytotechnology (Cytotechnology) Vol. 33 Issue 1-3 Pg. 247-51 (Jul 2000) ISSN: 0920-9069 [Print] United States
• PMID: 19002832 (Publication Type: Journal Article)