Apicidin decreases phospholipase C gamma-1 transcript and protein in Hut-78 T lymphoma cells.

Abstract

Phospholipase C gamma-1 (PLCgamma1) phosphorylation is a key step in intracellular signal transduction in T cells. We used Hut-78 T lymphoma cells to demonstrate the effect of apicidin on cellular levels of the PLCgamma1 molecule. Using reverse-transcription, real-time quantitative PCR and Western blot analysis, we observed that apicidin reduced the PLCgamma1 transcript and protein contents in Hut-78 T lymphoma cells. Our results indicate that protein synthesis appears to be crucial in the apicidin decrease of PLCgamma1 mRNA steadiness. Moreover, we determined that apicidin reduces the half-life of PLCgamma1 mRNAs from approximately 2 to 4h. Since PLCgamma1 is considered a key molecule in signal transduction in T cells, apicidin may be useful in the treatment of some autoimmune diseases in which autoreactive T cells occur.

• Authors: Szymon Debicki, Paweł P Jagodzinski
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 63 Issue 7 Pg. 543-7 (Aug 2009) ISSN: 1950-6007 [Electronic] France
• PMID: 18993024 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Histone Deacetylase Inhibitors
• Peptides, Cyclic
• RNA, Messenger
• apicidin
• Phospholipase C gamma

Topics

• Animals
• Blotting, Western
• Cell Line, Tumor
• Histone Deacetylase Inhibitors
• Lymphoma, T-Cell
• Peptides, Cyclic (pharmacology)
• Phospholipase C gamma (antagonists & inhibitors, biosynthesis, genetics)
• Phosphorylation
• Polymerase Chain Reaction
• RNA, Messenger (biosynthesis, genetics)
• Rabbits
• Signal Transduction (genetics)