Retention of phenylalanine ammonia-lyase activity in wheat seedlings during storage and in vitro digestion.
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| Abstract |
The retention of phenylalanine ammonia-lyase (PAL) activity in Red Spring wheat seedlings during storage and in vitro protein digestion was evaluated toward assessing the efficacy of plant PAL as a dietary supplement for patients suffering from the metabolic disease, phenylketonuria. Retention of PAL activity in freeze-dried wheat seedling tissues following three months of storage at -20 degrees C ranged from 62% in the leaf to 89% in root/residual seed tissues. After a 3-h two-stage ("gastric-intestinal") in vitro digestion, 36% and 42% recovery of PAL activity was associated with chopped fresh leaf and root/residual seed tissues respectively; however, no activity was recovered from freeze-dried tissues. High performance liquid chromatographic analysis of the residual phenylalanine (Phe) after in vitro digestion confirmed that the fresh tissues effected a significantly higher conversion of exogenous Phe than freeze-dried tissues. These results demonstrate that the plant cell walls provide protection of PAL during in vitro digestion. In cases where exogenous Phe (100 mg; 24 mM) was supplied to the tissues, the product of the reaction, trans-cinnamic acid, may have exerted a significant inhibitory effect on PAL activity.
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| Authors | M Lam, C H Scaman, S Clemens, A Kermode |
| Journal | Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 56 Issue 23 Pg. 11407-12 (Dec 10 2008) ISSN: 1520-5118 [Electronic] United States |
| PMID | 18989972 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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