Bacterial toxins induce heat shock proteins in human neutrophils.

Abstract

We studied the influence of different bacterial toxins (alveolysin; toxic shock syndrome toxin 1, TSST-1 and erythrogenic toxin A, ETA) on the expression of heat shock proteins (hsps) in isolated human polymorphonuclear granulocytes (PMNs). As was shown by Western blotting (anti-hsp72) ETA and TSST-1 were potent inducers of hsps at low toxin concentrations (10 ng/ml). Alveolysin led to the expression of hsps at hemolytic concentrations (1 HU; 700 ng/ml) whereas at subhemolytic concentrations (7 ng/ml) no heat shock response was observed. The induction of heat shock proteins was also accompanied by increased mRNA levels for hsp70 as was determined by PCR-analysis.

• Authors: T Hensler, M Köller, J E Alouf, W König
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 179 Issue 2 Pg. 872-9 (Sep 16 1991) ISSN: 0006-291X [Print] United States
• PMID: 1898407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Bacterial Toxins
• Enterotoxins
• Exotoxins
• Heat-Shock Proteins
• Hemolysin Proteins
• Membrane Proteins
• Organic Chemicals
• Pyrogens
• RNA, Messenger
• SpeA protein, Streptococcus pyogenes
• Superantigens
• enterotoxin F, Staphylococcal
• erythrogenic toxin
• alveolysin

Topics

• Bacterial Proteins
• Bacterial Toxins (pharmacology)
• Base Sequence
• Blotting, Western
• Electrophoresis, Polyacrylamide Gel
• Enterotoxins (pharmacology)
• Exotoxins (pharmacology)
• Gene Expression
• Heat-Shock Proteins (metabolism)
• Hemolysin Proteins (pharmacology)
• Humans
• Membrane Proteins
• Molecular Sequence Data
• Neutrophils (drug effects)
• Organic Chemicals
• Polymerase Chain Reaction
• Pyrogens (pharmacology)
• RNA, Messenger (metabolism)
• Staphylococcus aureus
• Superantigens