Abstract |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing.
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Authors | Jentaie Shiea, Yi-Tzu Cho, Yen-Hsiu Lin, Chin-Wen Chang, Li-Hua Lo, Yi-Chern Lee, Hung-Lung Ke, Wen-Jeng Wu, Deng-Chyang Wu |
Journal | Rapid communications in mass spectrometry : RCM
(Rapid Commun Mass Spectrom)
Vol. 22
Issue 23
Pg. 3754-60
(Dec 2008)
ISSN: 0951-4198 [Print] England |
PMID | 18980259
(Publication Type: Journal Article)
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Chemical References |
- Coumaric Acids
- Gentisates
- alpha-cyano-4-hydroxycinnamate
- sinapinic acid
- 2,5-dihydroxybenzoic acid
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Topics |
- Adult
- Albuminuria
(diagnosis)
- Coumaric Acids
- Female
- Gentisates
- Humans
- Male
- Middle Aged
- Sensitivity and Specificity
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
(methods)
- Young Adult
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