Abstract | BACKGROUND: METHODS: Blood samples were taken from eight active BD patients who showed an immune response to 40 mixed overlapping peptides spanning the entire sequence of human S-Ag. Peripheral blood mononuclear cells were isolated and stimulated with single S-Ag peptide at 5 microg/ml or 20 microg/ml. Single-cell immune responses were measured by IFN-gamma ELIspot assay. RESULTS: BD patients heterogeneously responded to the S-Ag peptides at two concentrations. In general, the responses to 5 microg/ml peptides were slightly stronger than those to 20 microg/ml peptides, while the maximum SFC frequency to single peptide at the two concentrations was similar. Several peptides including P31, P35 and P40 induced a prominent response, with the frequency of S-Ag specific cells being about 0.007%. Significant reactivity pattern shift was noted in patients with different disease courses. CONCLUSIONS: Certain active BD patients have S-Ag specific Th1 cells with a low frequency. The S-Ag epitope specificity between patients is highly heterogeneous, and varies with the uveitis course.
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Authors | Changlin Zhao, Peizeng Yang, Hao He, Xiaomin Lin, Liping Du, Hongyan Zhou, Aize Kijlstra |
Journal | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
(Graefes Arch Clin Exp Ophthalmol)
Vol. 247
Issue 4
Pg. 555-60
(Apr 2009)
ISSN: 1435-702X [Electronic] Germany |
PMID | 18958488
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Arrestin
- Autoantigens
- Immunodominant Epitopes
- Peptide Fragments
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Topics |
- Adult
- Amino Acid Sequence
- Arrestin
(immunology)
- Autoantigens
(immunology)
- Behcet Syndrome
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Epitope Mapping
- Humans
- Immunity, Cellular
- Immunodominant Epitopes
(immunology)
- Male
- Molecular Sequence Data
- Peptide Fragments
(immunology)
- Th1 Cells
(immunology)
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