The effect of low-intensity
laser radiation of the blue (441.2 nm), green (532 nm), and red (632.8 nm) spectral regions on the healing of experimental skin
wounds in rats has been studied. The effect of the traditionally applied
laser radiation in the red region has been compared with the effect of
laser radiation in the other spectral regions, assuming that, upon irradiation of
wounds by
lasers emitting in the blue and green regions, a similar effect can be achieved at lower doses. The following parameters characterizing the healing of experimental
wounds were used: the functional activity of phagocytes of
wound exudates, which was determined by
luminol-dependent chemiluminescence, and their number; the
antioxidant activity of
wound exudates; and the rate of healing, which was determined as a change in the
wound area. It was shown that irradiation with
laser accelerated the healing of
wounds in all cases. The exposure to
laser radiations in the red (1.5 J/cm), blue, and green (0.75 J/cm2) spectral regions shortened the time of wound healing from 22 to 17 and 19 days, respectively. The functional activity of leukocytes after the exposure increased on day 5 after the infliction of the
wound, whereas in the control it decreased. The
superoxide dismutase activity increased in all experimental groups by day 5 after the operation. A maximum increase in the
superoxide dismutase activity occurred after the exposure to
laser radiation in the red region at a dose of 1.5 J/cm and in the blue and green spectral regions at a dose of 0.75 J/cm2.