Abstract | BACKGROUND: METHODS: After immediate post- burn infection with P. aeruginosa, mortality and morbidity (daily weight changes) were monitored in mice treated with anti-fla-a as compared to untreated mice. Bacterial dissemination and invasiveness were monitored by bacterial counts at the burn site and spleen. Three different timing regimens for anti-fla-a treatment were studied: (a) prophylaxis (pre- infection), (b) therapeutic (post- infection), and (c) combined mode. RESULTS: Combined regimen of anti-fla-a markedly improved survival of mice infected with P. aeruginosa from 6% to 96% (p<0.0001), similar to treatment with Imipenem. Furthermore, a significant improvement in survival was obtained when anti-fla-a was given prior to (75% survival) or post- infection (50% survival). It reduced bacterial load in the spleen (p=0.01), preventing bacterial sepsis. CONCLUSION: Anti-fla-a is effective in reducing mortality and morbidity in murine P. aeruginosa-infected burn model.
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Authors | Yoav Barnea, Yehuda Carmeli, Lewis F Neville, Hamutal Kahel-Reifer, Rachel Eren, Shlomo Dagan, Shiri Navon-Venezia |
Journal | Burns : journal of the International Society for Burn Injuries
(Burns)
Vol. 35
Issue 3
Pg. 390-6
(May 2009)
ISSN: 1879-1409 [Electronic] Netherlands |
PMID | 18951715
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Flagellin
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Topics |
- Animals
- Antibodies, Monoclonal
(therapeutic use)
- Antibody Specificity
- Burns
(drug therapy, microbiology)
- Female
- Flagellin
(immunology)
- Immunohistochemistry
- Mice
- Mice, Inbred C57BL
- Pseudomonas Infections
(drug therapy, immunology)
- Pseudomonas aeruginosa
(immunology)
- Sepsis
(drug therapy, microbiology)
- Skin
(pathology)
- Wound Infection
(microbiology)
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