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Therapy with anti-flagellin A monoclonal antibody limits Pseudomonas aeruginosa invasiveness in a mouse burn wound sepsis model.

AbstractBACKGROUND:
The aim of this study was to evaluate the effect of an anti-flagellin sub-type monoclonal antibody (anti-fla-a) on Pseudomonas aeruginosa infection in a mouse burn model and to assay bacterial dissemination and invasiveness.
METHODS:
After immediate post-burn infection with P. aeruginosa, mortality and morbidity (daily weight changes) were monitored in mice treated with anti-fla-a as compared to untreated mice. Bacterial dissemination and invasiveness were monitored by bacterial counts at the burn site and spleen. Three different timing regimens for anti-fla-a treatment were studied: (a) prophylaxis (pre-infection), (b) therapeutic (post-infection), and (c) combined mode.
RESULTS:
Combined regimen of anti-fla-a markedly improved survival of mice infected with P. aeruginosa from 6% to 96% (p<0.0001), similar to treatment with Imipenem. Furthermore, a significant improvement in survival was obtained when anti-fla-a was given prior to (75% survival) or post-infection (50% survival). It reduced bacterial load in the spleen (p=0.01), preventing bacterial sepsis.
CONCLUSION:
Anti-fla-a is effective in reducing mortality and morbidity in murine P. aeruginosa-infected burn model.
AuthorsYoav Barnea, Yehuda Carmeli, Lewis F Neville, Hamutal Kahel-Reifer, Rachel Eren, Shlomo Dagan, Shiri Navon-Venezia
JournalBurns : journal of the International Society for Burn Injuries (Burns) Vol. 35 Issue 3 Pg. 390-6 (May 2009) ISSN: 1879-1409 [Electronic] Netherlands
PMID18951715 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Flagellin
Topics
  • Animals
  • Antibodies, Monoclonal (therapeutic use)
  • Antibody Specificity
  • Burns (drug therapy, microbiology)
  • Female
  • Flagellin (immunology)
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Pseudomonas Infections (drug therapy, immunology)
  • Pseudomonas aeruginosa (immunology)
  • Sepsis (drug therapy, microbiology)
  • Skin (pathology)
  • Wound Infection (microbiology)

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